Isolation of strains from different objects or materials, and obtaining of pure cultures 

At the Microbial Culture Collection Ex, we can perform on demand microbiological overviews of infected samples of food, drink, feed, seeds, packaging, industrial products, and other materials. However, we do not isolate anything from human tissues or other human samples. Microorganisms isolated from submitted materials can be identified to the genus or species level. and after finalisation of the work, a report is sent to the customer. Microorganisms can be also isolated from different natural samples, such as soil, air, water, among others. 



Identification and characterisation of strains 

Correct identification of microorganisms is of utmost importance for scientific research, phytopathology, industrial contamination, and many other aspects. At the Microbial Culture Collection Ex, unknown microorganisms are taxonomically identified based on their macroscopic, microscopic, biochemical and molecular characteristics. Since the establishment of the Microbial Culture Collection Ex, in total around 5,000 fungal and 460 bacterial cultures have been identified. 


Yeast are identified based on sequencing of the ITS or 28S rDNA, while filamentous fungi are identified based on morphological characteristics of the culture on selected media and sequencing of the ITS rDNA. In the Microbial Culture Collection Ex, we also save sequence data of type strains and other well-characterised strains, which can be used to help with identification. Occasionally, we can describe microorganisms new to science, in agreement with the depositor. Upon request, we can provide macroscopic or microscopic pictures of the strains of microorganisms identified and described in the Microbial Culture Collection Ex, including microphotographs obtained through either transmission or scanning electron microscopy.